CsCl-EtBr plasmid prep.

VANNESTEJ%RUASV1 at ruakura.cri.nz VANNESTEJ%RUASV1 at ruakura.cri.nz
Thu Dec 2 05:11:41 EST 1993


Kamal wrote:
Hi, Here is the method that always works for me.
Remove the band witha syringe
Extract 2-3 times with water saturated butanol
dilute the EtBr free aqeous phase 3 fold with TE to avoid the ppt.of CsCl in
a corex tube
add NaCl to 0.1M or NaAc to 0.3M, mix
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Due to the high concentration of CsCl, it works fine without adding any salt.  

Cheers,

Joel



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