increasing amplification by 2 rounds of PCR

BCRUBIN at weizmann.weizmann.ac.il BCRUBIN at weizmann.weizmann.ac.il
Wed Dec 1 12:16:36 EST 1993


Hi netters.
  I have being playing with nested PCR for somtime now. You might have a real p
roblam of single primer amplification. If you just use 2 rounds of amplificatio
n, all the "mistakes" (or FLASE POSITIVES) of the first round would be amplifie
d in the second. Nested PCR reduces the chances of that.
      Eitan.



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