Exo III Deletions
bturner at MED-ITVAX1.BU.EDU
Fri Dec 3 02:39:28 EST 1993
Does any one have any experience with the Exo III deletion
method of W Richard McCombie et al. published in
METHODS(A companion to Methods in Enzymology) 3(1):33-40, 1991 ?
This method recommends a Klenow end repair/blunting of the
deleted plasmid both before and after an agarose gel purification
of the deleted plasmid.
Why is the second repair reaction done? Is it necessary or
redundatn? What is its effect on the subsequent ligation and
bturner at med-itvax1.bu.edu
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