XGal stining and IPTG

GWDGV1::KCHOWDH kchowdh at gwdgv1.dnet.gwdg.de
Thu Dec 2 07:32:35 EST 1993


Hi Netter,
In the bacterial system IPTG is used because the promoter is IPTG inducible.
In the eucaryotic system the promoter does not depend on IPTG induction. So
for example with LacZ constructs with LTR or SV40 promoters or in transgenic
mice containing the LacZ gene under the control of a cellular promoter, there
is no need for IPTG.
Cheers,
Kamal Chowdhury
Max Planck Institute
Am Fassberg
37077 Goettingen
germany



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