davidian at msdos.montpellier.inra.fr
Thu Dec 2 06:56:32 EST 1993
Question about guanidium thiocyanate extraction of RNA
I have just done an RNA extraction on plant tissue using the
method basically as described in Current protocols in
Mol.Biol only using a polytron to grind up the tissue. The
only problem was that as there was sarkosyl in the
extraction buffer there was a huge amount of froth after
only a few seconds - is it possible to leave the sarkosyl
out and add it after homogenising or is there is a knack
to using a polytron?
Thanks in advance for any advise.
Davidian at montpellier.inra.fr
Biochimie et Physiology Vgtales
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