?RNA extractions

DAVIDIAN davidian at msdos.montpellier.inra.fr
Thu Dec 2 06:56:32 EST 1993


          Question about guanidium thiocyanate extraction of RNA

          I have just done an RNA extraction on plant tissue using the
          method basically as described in Current protocols in
          Mol.Biol only using a polytron to grind up the tissue. The
          only problem was that as there was sarkosyl in the
          extraction buffer there was a huge amount of froth after
          only a few seconds - is it possible to leave the sarkosyl
          out and add it after homogenising or is there is a knack
          to using a polytron?

          Thanks in advance for any advise.

          Helen

          Davidian at montpellier.inra.fr
          Biochimie et Physiology Vgtales
          ENSA-M/INRA
          Montpellier.



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