E.coli JC8679

Dan Jacobson danj at dev.gdb.org
Thu Dec 2 11:13:18 EST 1993

In article <753993749snz at genesys.demon.co.uk> Duncan at genesys.demon.co.uk writes:
>Hi Netters,
>Does anyone have the E.coli strain JC8679 as used for in vivo cloning of
>PCR products in E.coli, NAR, Vol 21 No. 22, pp5192-5197. Failing that an 
>Email address for Bert Vogelsteins's Lab at The John Hopkins Oncology Centre
>at Baltimore.
> ....

A little gopher search of the ATCC data finds the following about
E. coli strain JC8679:


Escherichia coli
ATCC 47001


H. Okayama JC8679. Genotype: F- thr-1 leuB-6 phi-1 lacY1 galk2
ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 supE44 recB21
recC22 sbcA23 his-328 lambda-. Host for propagation of lambdaDNA
libraries. J. Bacteriol. 145: 521-531, 1981; Mol. Cell. Biol. 5:
1136-1142, 1985.

Growth Conditions: Medium 1065  37C

Shipped: Freeze-dried.

Price Code: C


To find out more about the ATCC, do your own searches, place an order 
etc. - point your gopher client at gopher.gdb.org and select:

 -->  12. Search Databases at Hopkins (Vectors, Promoters, NRL-3D, EST, OMIM../

  -->  6.  Culture Collections (ATCC, ECACC)/


I don't know Vogelstein's e-mail address offhand but his phone number is:
(410) 955-8877.

Best of luck,

Dan Jacobson

danj at mail.gdb.org

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