blue X-gal stocks

John Cooper picard at nih.gov
Thu Dec 2 11:24:14 EST 1993


In article <leach-011293221424 at med-pharm5.bu.edu> leach at mbcrr.harvard.edu (Martin Leach) writes:
>Path: nih-csl!uunet!olivea!news.bu.edu!med-pharm5.bu.edu!user
>From: leach at mbcrr.harvard.edu (Martin Leach)
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: Re: blue X-gal stocks
>Followup-To: bionet.molbio.methds-reagnts
>Date: Wed, 01 Dec 1993 22:14:24 +0000
>Organization: Boston University Dept. of Pharmacology
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>Distribution: world
>Message-ID: <leach-011293221424 at med-pharm5.bu.edu>
>References: <2dj72p$2s3 at samba.oit.unc.edu>
>NNTP-Posting-Host: med-pharm5.bu.edu
>In article <2dj72p$2s3 at samba.oit.unc.edu>, jtsuruta at med.unc.edu (James
>Tsuruta) wrote:
>
>> I'm posting this for someone next-door.  Has anyone ever seen their X-gal
>> stock in DMF turn pale blue?  What can cause this? and is it OK to use in
>> top agar?
>
>our X-gal goes brown/orange when overexposed to light.
>
>
>Martin Leach
>
>
>-- 
>
>.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
>   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
>   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
> _/  |-/__==/  80 E. Concord St. (L603)
>(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
>               USA                           head.....WIBBLE" -BLACKADDER  

We use our xgal that has turned yellow on our top agar and it works fine...
John Cooper, MD
NIH-NHLBI-LCM
Picard at Helix.NIH.GOV
"The views expressed are mine, not my boss's... That's what she pays me for..."



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