blue X-gal stocks

John Cooper picard at
Thu Dec 2 11:24:14 EST 1993

In article <leach-011293221424 at> leach at (Martin Leach) writes:
>Path: nih-csl!uunet!olivea!!!user
>From: leach at (Martin Leach)
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: Re: blue X-gal stocks
>Followup-To: bionet.molbio.methds-reagnts
>Date: Wed, 01 Dec 1993 22:14:24 +0000
>Organization: Boston University Dept. of Pharmacology
>Lines: 21
>Distribution: world
>Message-ID: <leach-011293221424 at>
>References: <2dj72p$2s3 at>
>In article <2dj72p$2s3 at>, jtsuruta at (James
>Tsuruta) wrote:
>> I'm posting this for someone next-door.  Has anyone ever seen their X-gal
>> stock in DMF turn pale blue?  What can cause this? and is it OK to use in
>> top agar?
>our X-gal goes brown/orange when overexposed to light.
>Martin Leach
>.....          Martin Leach                Email:leach at 
>   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
>   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
> _/  |-/__==/  80 E. Concord St. (L603)
>(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
>               USA                           head.....WIBBLE" -BLACKADDER  

We use our xgal that has turned yellow on our top agar and it works fine...
John Cooper, MD
Picard at Helix.NIH.GOV
"The views expressed are mine, not my boss's... That's what she pays me for..."

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