Lambda DNA mini-preps?

Richard Near rocket1 at bu.edu
Sat Dec 4 18:34:15 EST 1993


If someone has a good method to prepare lambda DNA as minipreps, I'd
be interested.  I've been growing 50ml (using LE392), doing a PEG
precip., proteinase K, Phenol/Chloroform technique. I've been getting
low yields, but the DNA is at least cuttable.

  What can I do to increase my yields (I get good lysis) yet keep the 
prep simple.  The DNA should be clean enough for clone mapping and
I intend to use it as a template for PCR sequencing.

Mucho thanks,
Rick



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