Help:How to inactivate Viper venom

Paul Riggs riggs at neb.com
Fri Dec 3 15:18:39 EST 1993


Hi Dan

We immobilize the viper venom protease on agarose beads, and then run a
Sephadex G-100 or equivalent (the MW of the viper venom protease is ~105
kD) in case any came off the beads. The venom protease is completely
inactive without Ca++, so EDTA works well as a reversible inhibitor. A good
reference (if your library goes back far enough) is Schiffman et al. 1969,
Biochemistry 8(4)1397-1405.

Paul

In article <dkolk-241193165231 at clinical-mac-95.ucsd.edu>,
dkolk at popmail.ucsd.edu (Dan Kolk) wrote:
> 
> 	We are doing our own Factor X activation with Russells viper venom.  We
> have yet to find an article describing how to inactivate the viper venom
> once factor X is activated.  Anyone know any good articles on the subjects.
> 
> 																																			Thanks in advance 
> 																																				Dan Kolk

______
Paul Riggs
New England Biolabs
riggs at neb.com



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