Good Plasmid software required

[Jim Owens]

In article <1993Dec2.145039.13325 at alw.nih.gov> Jim Owens,
jow at helix.nih.gov writes:
>The only drawback, beyond the price, is that it requires that the
>sequences of the fragments and vectors be known.

Bob Gross, the creator of Gene Construction Kit, tells me there is a
simple work around for a plasmid whose restriction map is known but whose
sequence is unknown or partially known:

Fill in the sequence with N's for the unknown bases between sequences for
known restriction sites.

Good luck,

Jim Owens