ptn ppt problem

Shahram Mori smori at nmsu.edu
Sat Dec 4 23:02:01 EST 1993


Dear netters,
I have been ammonium sulfate pptating cyanobacterial cell extracts and
resuspending the pellet in PBS. When I boil my sample before loading onto
a gel, there is a lot of precipitate. I have no idea where this ppt is
coming from. My loading buffer has 312.5mM Tris7.2, 50% glycerol, B-mercap,
1.0% SDS and  Pyronin Y.
If you have any clue I would very much appreciate it.
Thanks.
Shahram



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