przemko at reks.uia.ac.be
Mon Dec 6 09:31:52 EST 1993
Yeah, spermidine...Anyone can help me how to get rid of it?
I am doing in vitro transcriptions using Megascript (Ambion).
The kit works great (80-100 ug/ug of the template) but the
resulting RNA is not always soluble. I suspect spremidine
(included in their "secret" buffer). Normal kit makes RNA
that I can concetrated to the same level (10ug/ul) without
any particulate matter being visible.
So, any help will be greatly appreciated
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