His-tagged (Ni-Agarose) Purification of Proteins

Seth Findley findley at carson.u.washington.edu
Tue Dec 7 16:40:38 EST 1993

Does anybody out there have any improvements/tips for using the 
his-tagged protein Ni-agarose technique of proteins?
I am using the Ni-resin from Qiagen and the recommended strains of E.coli
but am getting low expression and degradation...

Any help would be appreciated!


Seth Findley
University of Washington

More information about the Methods mailing list