His-tagged (Ni-Agarose) Purification of Proteins

Seth Findley findley at carson.u.washington.edu
Tue Dec 7 16:40:38 EST 1993


Does anybody out there have any improvements/tips for using the 
his-tagged protein Ni-agarose technique of proteins?
I am using the Ni-resin from Qiagen and the recommended strains of E.coli
but am getting low expression and degradation...

Any help would be appreciated!

thanks,

Seth Findley
University of Washington
Seattle



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