Detection of Carbohydrate

Klara Briknarova kb87+ at andrew.cmu.edu
Wed Dec 8 20:01:59 EST 1993


I work with a small glycoprotein. I would like to enzymaticaly remove
the carbohydrate and then separate the carbohydrate and the protein
moiety (using chromatography). I am looking for a method sensitive
enough to detect 0.01 - 0.1 mg of the released carbohydrate.
This is my carbohydrate:
-GlcNac(-Fuc)-GlcNac-Man-(Man-GlcNac-Gal-SA)2

If you have any hints, suggestions, or PROTOCOLS I will appreciate it.

               Thanks.
                                        Klara



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