Recovery of DNA from acrylamide gels

Helge Weissig helgew at LJCRF.EDU
Wed Dec 8 17:23:41 EST 1993


pnh at fcsparc6.ncifcrf.gov (Paul N Hengen) wrote:

>| Does anyone have an efficient method of recovering DNA fragments from 
>| acryalmide gels, size of the order of 4.5kb?
>
>: I am going to collect 93bp PCR product from agarose gel. Could you tell me
>: the method Dr. Weissig put in this newsgroup is suitable or not?  
>
>I think you guys have it all backwards! I would recover the 4.5 kb
>fragment from a 1.0 % agarose gel and the 93 bp fragment from a
>6-7.5 % polyacrylamide gel.
>
[stuff deleted]

dito! :)

cheers,
helge


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