What conc. HCl needed to depurinate DNA?
bhandary at wccf.mit.edu
Wed Dec 8 16:46:00 EST 1993
In article <93340.175216RROXBY at MAINE.MAINE.EDU>, Robert Roxby <RROXBY at MAINE.MAINE.EDU> writes...
>I want to make sure some glasssware is free of contaminating
>DNA and would appreciate being pointed to the appropriate
>reference. Thanks, Bob
For depurinating dna is agarose gels, I use 0.1M HCL for 10 minutes. I would
think that this concentration would work on glassware. Perhaps be on the safe
side and use 0.2N for 30 minutes. Follow by 0.2N NaOH to break the chain if
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