degenerate oligo design

Jonathan Gary jgary at
Wed Dec 8 13:26:48 EST 1993

To: Tom MacAllister:

This is not really an answer, yet.   But have you thought about using the
20mer, implementing codon bias?  Although you might select out the ONE
right sequence, it does have the advantage of allowing you to use the
longer primer and lowering the overall degeneracy.

Otherwise, I'd probably use the 17mer and all combinations.

        Jonathan D. Gary                      Savoir Faire
        UCLA                              (in the BOLO community)
        Molec. Biol. Instit.    //////////////////////////////////
        3rd yr Grad Stud.       \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\

More information about the Methods mailing list