ptn question?

Shahram Mori smori at
Sat Dec 4 21:38:38 EST 1993

Dear netters,
I have been doing western blots on ammonium sulfate ppted Cyanobacterial cell
crude extracts. My loading buffer has Pyronin y, 312.5mMTris 7.2, 50%glycerol
1.0% SDS, 2-Bmercap. and appropriate amount of protein. After boiling my 
sample for 10 minutes before loading I see a massive amount of ppt,
Can anyone tell me what this ppt is? If it has my ptns in there, i am toast.
Thanks for your response.
e-mail smori at

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