single-stranded bridge repair

mtsman07 at uctvax.uct.ac.za mtsman07 at uctvax.uct.ac.za
Fri Dec 10 08:55:55 EST 1993


Dear netters

I have been trying to clone an oligonucleotide into pUC 19 derivative plasmid
pWM 529 using Mandecki`s single-stranded bridge-repair protocol. I have 
followed the protocol  very carefully but still have very little recombination
if at all. Does anyone out ther have some advice?



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