RNA isolation

tristan zand zand at medsun.unige.ch
Fri Dec 10 03:52:08 EST 1993


We have some important blood samples at -80C: about 10 ml whole blood, in a rather strange
solution:

1.8 M guanidine
5.8 mM Na citrate
20 mM B-ME
0.12 % lauryl sarcosine

Our first attempts to get RNA out failed: we tried adding guanidine to 4M and CsCl to 2.37 M,
then spinning it through a CsCl step: got nothing!!!!!!!

Any ideas how we can get enough RNA for RT-PCR out of these frozen samples?

Could you answer by E-mail to young at cmu.unige.ch

Michele Young



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