sequencing gel problems

Don Demuth demuth at biochem.dental.upenn.edu
Fri Dec 10 15:11:12 EST 1993


In article <9312101656.AA14841 at net.bio.net>, N490047 at UNIVSCVM.CSD.SCAROLINA.EDU (Tom) writes:
|> Dear Netters-
|>       We are having a problem with increasing voltage/decreasing amperage
|> during our pre-run.  As a result, we cannot maintain high enough wattage.  We
|> have changed all of the reagents in the gel and buffer (new bottles) and
|> nothing seems to be wrong with our aparatus (BRL #S2 with LKB 2103 power
|> supply).  We have also tried TBE from another lab with the same result.
|> Suggestions?
|> 
|>             Tom



	Ran into the same problem myself several years ago and couldn't figure
out what was wrong for days.  Changed solutions, tried other's reagents,
all that stuff.  Finally found that the technician was de-ionizing
the acrylamide solution *after* adding the TBE.  Does wonders for the buffering
capacity of the TBE and the electrical properties of the gel.


			DRD  
-- 

 

    _/_/_/_/   _/_/_/_/   _/    _/   _/    _/  Donald R. Demuth
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