ptn ppt problem

Bill Nowatzke wnowatzk at ucs.indiana.edu
Sat Dec 11 09:53:43 EST 1993


I too often see a ppt when placing high salt column fractions in SDS PAGE
sample buffer. I think that the ppt is due to the salt causing the SDS to
ppt, as one method of staining an SDS PAGE gel is to immerse it in 0.25M
KCl and the SDS ppt. Anyway, it sounds like you are getting ready to load
a gel, simply leave the sample in your heating block just until you load
your lane, then load it quickly.



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