How to purify single stranded DNA fragments?

Bruce Roe broe at aardvark.ucs.uoknor.edu
Mon Dec 13 07:31:00 EST 1993


In article <16908.jdboer at sol.uvic.ca>, <jdboer at sol.UVic.CA> writes...
>Hi,
> 
>I want to purify single stranded DNA fragments of about 1 kb in length. Is 
>anyone aware of some small easy to use little column that would do this. 
>As I know, most small columns work fine with ds DNA fragments, but may not 
>do so well with single stranded fragments. I want to avoid doing gels, 
>phenol extractions or ethanol precipitations since I have several thousand
>samples waiting. Any ideas?
> 
>Johan de Boer
>jdboer at sol.Uvic.CA
>Victoria, Canada

And you want to use columns for "several thousand samples"??  Get real.
Check out these four papers, the first three describe automation of the
fourth with minor modifications.  

1. S. L. Chissoe, Y.F. Wang, S.W.  Clifton, N. Ma, H.J. Sun, J. S.Lobsinger,
   S. M. Kenton, J. D. White and  Roe, B.A. (1991). Methods: A Companion to
   Methods In Enzymol. 3, 55-65.

2. Smith, V., Brown, C.M., Bankier, A.T., and Barrell, B.G. (1990) DNA 
   Sequence 1, 73-78.

3. Mardis E.R. and Roe, B.A. (1989). BioTechniques  7, 736-746.

4. Eperon, I.C. (1986).  Anal. Biochem. 156, 406-412.

Cheers.......bruce
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