Bacterial ennum. by PCR

MARK DOWE dowe at ace.acadiau.ca
Mon Dec 13 23:12:49 EST 1993


Hi all,

     I'm working on a procedure to enumerate a species of bacteria in soil 
using PCR, and I was wondering if anyone had any experience/advice that 
they would be willing to share?
     Specifically, I'm working with Listeria monocytogenes and primers for 
the listeriolysn gene.  I was hoping to use the procedure in studies of a 
possible viable but nonculturable state in the organism. 



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