DEPC

[chai_z at wehi.edu.au]

In article <2D0C3BC0.554 at news.service.uci.edu>, rwcitek at orion.oac.uci.edu (Robert Citek) writes:
> I need to work with RNA and have looked in several sources to 
> minimize or eliminate RNAse contamination.  DEPC was recommended
> in 0.2%, 0.1%, 0.5%, and 0.001% concentrations to treat my water 
> for preparing buffers.  I've never worked with RNA before.  Does 
> it really matter what concentration I use?  That is, is 0.2% going
> to be so high that it will interfere with later RNA use or is 0.001%
> going to be so low as to be ineffective?
> 

I used 0.1%, incubated at 37 degree C o/n and autoclaved. It worked well.


Zhonglin Chai
Melbourne
















> Thanks in advance for any and all advice.
> 
> ----------------------------------------------------------
> Robert Citek
> University of California
> 134 Social Ecology
> Irvine, CA  92717-5150
> rwcitek at uci.edu