QuickHyb/Immed. Reply pls!

at reks.uia.ac.be at reks.uia.ac.be
Thu Dec 16 03:32:43 EST 1993


In article <1993Dec15.152205.19494 at alw.nih.gov> dspete at helix.nih.gov writes:
>
>In article <2elfat$dn1 at news.mic.ucla.edu>, <jgary at ewald.mbi.ucla.edu> writes:
>> Hey netters:
>> 
>> I was wondering if any of you have used Quickhyb (Stratagene) for S.
>> blots????More specifically.....has anyone used it with Millipore's
>> Imobilon-N membrane????
>> results comments
>> requested...........>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<
>> a quick reply would really help me 
>> 
>> thanx folks....
>> 
>> Jon Gary
>> UCLA
>> 3rd year grad
>> Mol. biol. inst.
>
>Jon,
>
>I've used QuikHybe with several different membranes, though Immobilon-N was not 
>among them. Works great with everything I've tried.
>
>-------------------------------------------------------------------------
>* David S. Peterson, Ph.D.          Phone  (301) 496-4023               *
>* National Institutes of Health                                         *
>* Laboratory of Malaria Research    Fax    (301) 402-0079               *
>* Bldg4, Rm B1-34                                                       *
>* Bethesda MD, 20892                Email: dspete at helix.nih.gov         *
>-------------------------------------------------------------------------
>
>
We used QuickHyb (Stratagene) and RapidHyb (Amersham).
The former is about 5 times better (in terms of time, the
quality is the same). The latter is about 4 times cheaper.
So, I guess depends what you want. For routine work we use
RapidHyb, for a REALLY fancy one, QuickHyb

Przemko Tylzanowski Ph.D.
Dept. Biochemistry
University of Antwerp



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