pulse-chase in E. coli

David Kling dkling at warren.med.harvard.edu
Thu Dec 16 14:20:34 EST 1993


Hello netters,

I would like to know whether anybody has had any experience in doing
pulse-chase experiments in E. coli using the T7 polymerase overexpression
system described by S. Tabor and F.W. Studier.  I have had trouble chasing
out the label after a 1hr incubation at 37 degrees.  I would appreciate any
useful information that you may have.  Please send responses directly to my
E. mail address.  I will post all the responses together.  

My E. mail address is dkling at warren.med.harvard.edu.

Thank you in advance!



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