pulse-chase in E. coli

[David Kling]

Hello netters,

I would like to know whether anybody has had any experience in doing
pulse-chase experiments in E. coli using the T7 polymerase overexpression
system described by S. Tabor and F.W. Studier.  I have had trouble chasing
out the label after a 1hr incubation at 37 degrees.  I would appreciate any
useful information that you may have.  Please send responses directly to my
E. mail address.  I will post all the responses together.  

My E. mail address is dkling at warren.med.harvard.edu.

Thank you in advance!