rbritten at darby.bio.uci.edu
Sat Dec 18 15:14:42 EST 1993
I was hoping the world at large could help me with this
conundrum. I have this clone (6.6 kb) which encodes a
protein with an interesting domain . This gene lies at bases 1200-2200
and I thought there was a whole lot of non-coding region in this
cDNA clone but amazingly I learned a couple days ago that the
first 1000 bases encode a mitochondrial protein (and with mitochondrial
codon usage). I thought it was a cloning artifact and was about to
toss it when I realized that as a probe for a Northern a 1000 base 5'
end chunk of it identified a 6.6 kb (that's how big the cDNA is ) band.
Also, a prior partial cDNA way at the 3' end also yields a 6.6 kb
band on a Northern. Since the mito genome is so well characterized,
it is unlikely that the 1200-2200 gene is a novel mito. gene.
So, is this a trans-organellar transcript or what??
Do NOT send replies to my boss but rather to me at
tomm at mirsky.caltech.edu
or just post them here . Thanks.
P.S. The 1200-2200 encoded protein is real,it has a homologue in Dros,etc.
Also, it seems to be nuclear codon usage since AGG in torpedo
mito codons would (I think) put stop codons in the otherwise
open reading frame.
More information about the Methods