PCR contamination / sources of contamination

Linn, Charles Ernest linn at utkvx.utk.edu
Sat Dec 18 23:04:00 EST 1993


In article <vancleve-171293153256 at cbmac1.bdrc.bd.com>, vancleve at bdrc.bd.com (Mark Van Cleve) writes...
>What about growing the polymerase in a UDG deficient bug? The bug will have
>some uracil in its DNA as a result of deamination of cytosine, so, when you
>isolate your polymerase, you can destroy any contaminating DNA by treatment
>with UDG. The idea's from a Cetus patent application, I can look it up if
>anyone's interested.
> 
>-- 
>Mark Van Cleve
>Becton Dickinson Research Center
>Research Triangle Park, NC 27707
> 
>Any expressed opinions are not necessarily those of Becton Dickinson

Sounds like that would be a good possibility for producing enzyme relatively
free of contaminating DNA.  I'd certainly be interested in the reference.

Charles Linn
linn at utkvx.utk.edu
cel at howell.bio.utk.edu




More information about the Methods mailing list