PCR contamination / sources of contamination

Linn, Charles Ernest linn at utkvx.utk.edu
Sat Dec 18 23:04:00 EST 1993

In article <vancleve-171293153256 at cbmac1.bdrc.bd.com>, vancleve at bdrc.bd.com (Mark Van Cleve) writes...
>What about growing the polymerase in a UDG deficient bug? The bug will have
>some uracil in its DNA as a result of deamination of cytosine, so, when you
>isolate your polymerase, you can destroy any contaminating DNA by treatment
>with UDG. The idea's from a Cetus patent application, I can look it up if
>anyone's interested.
>Mark Van Cleve
>Becton Dickinson Research Center
>Research Triangle Park, NC 27707
>Any expressed opinions are not necessarily those of Becton Dickinson

Sounds like that would be a good possibility for producing enzyme relatively
free of contaminating DNA.  I'd certainly be interested in the reference.

Charles Linn
linn at utkvx.utk.edu
cel at howell.bio.utk.edu

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