Alkaline transfers - a possible variant?
mkennedy at chmeds.ac.nz
Mon Dec 20 18:09:23 EST 1993
In article <CIBvJy.2Dr at biobase.aau.dk>, pamaga at biobase.aau.dk (Paulo Magalhaes) writes:
> For all our transfers (DNA to Hybond) we use 0.4M NaOH.
> Since we use quite a lot of this, we get all these solutions
> made for us. By mistake we got a few litres of 0.4M NaOH, 0.6M NaCl.
> We don't really want to throw out all this, so we started wondering
> what COULD happen if we used this "new" solution for the transfer.
We use 0.4M NaOH, 0.6M NaCL routinely for our alkaline blots onto Hybond N, so
I'm sure it will work fine. In fact, I now use this as my universal denaturing
solution - for blots, phage lifts and stripping filters.
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-0750
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