Problem with BrdUr labelling
Chia Jin Ngee
mcblab47 at leonis.nus.sg
Tue Dec 21 02:52:09 EST 1993
Hi to all in Bionetland!
I have this current problem with BrdUr labelling and it is as follows:
1. I use Amersham's Cell Proliferation kit and instead of using the
anti-mouse peroxidase in the kit, I use anti-mouse FITC from BM to stain
the anti-BrdUr antibody from the kit. I notice background staining in the
cytosol of non-S phase CEM cells.
2. Instead of using the anti-BrdUr antibody supplied in the kit, I tried
the Oncogene Science anti-BrdUr FITC but unable to get any staining at
all. I followed the protocol from the Amersham kit.
Kindly advise on the above two problems.
Blessings and thanks
Jin Ngee, Chia Chemical Carcinogenesis Laboratory
(Genie, the OligoMan) Institute of Molecular and Cell Biology
mcblab47 at leonis.nus.sg National University of Singapore
Tel: (065)772-3797 Kent Ridge Crescent
Fax: (065)779-1117 Singapore 0511
"I'm Mr Chia not Ms Chia and I know Genie is a girl's name"-Jin Ngee, Chia
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