In vitro transcription/translation
Mike Lumb x3391
mlumb at crc.ac.uk
Tue Dec 21 11:22:19 EST 1993
I've got a problem with translation of RNA transribed from clones
in pBluescript KS+. RNA transcribed from the T3 promoter is
translated efficiently, RNA transcribed from the T7 promoter is
translated inefficiently or not at all! My transcripts are capped and
I translate in retic lysate.
Have I got a problem with polylinker sequence? Stratagene havent heard
of a problem, does anyone out there know better?
MRC Clinical Research Centre
email mlumb at crc.ac.uk
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