RT-PCR from Paraffin Embedded Tissue

LICHY at EMAIL.AFIP.OSD.MIL LICHY at EMAIL.AFIP.OSD.MIL
Thu Dec 16 11:44:53 EST 1993


        I would be interested in hearing from anyone with experience doing 
RT-PCR on extracts of fixed, paraffin embedded tissue.  What are the effects of 
the fixative used, length of fixation, age of block, method of storage, method 
of RNA purification, etc.?

Jack H. Lichy
Dept. of Cellular Pathology
Armed Forces Institute of Pathology
E-mail: lichy at email.afip.osd.mil



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