Site directed mutagenesis

Richard Near rocket1 at
Fri Dec 24 02:07:22 EST 1993


I usually do mutagenesis using the method of Kunkel
and uridinylated template (a BioRad kit).  I had
some trouble with secondary structure and coplettion
of second strand synthesis.  I found that using 
gene32 protein (sold by BioRad or Boehringer) 
seemed to help.  I'm curious if it would help you.
Also maybe trying to put the plasmid back into 
Rec- strains might help.

Good luck,
rocket1 at

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