CsCl purification of plasmid DNA

Mark Mark
Wed Dec 22 22:46:14 EST 1993


In article <2dgb1o$4tp at canopus.cc.umanitoba.ca> jrichmn at ccu.umanitoba.ca (Joy Richman) writes:
>Dear Netters,
>
>I have been having an intermittant problem with my Maxi preps. We are
>purifying plasmid DNA on a CsCl/ EtBr gradient in an ultracentrifuge,
>pulling the bands and then extracting the EtBr with TE saturated
>sec-butanol. Unfortunately we cannot seem to find our DNA after
>precipitation with 2 volumes of 100% EtOH. The DNA does not seem to want
>to come out of solution. We have tried adding 1/10th volume of 3 M NaOAc
>(pH 5.2) but no success. Can anyone suggest a reliable extraction
>procedure that will result in DNA pellets at the end of the prep?
>
>Joy Richman
>
>jrichmn at cc.umanitoba.ca
>
>-- 
>The most important event in life is not birth or death but gastrulation.
>Lewis Wolpert
>jrichmn at ccu.umanitoba.ca
>



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