Increased Transcription Yields

Dave Hall DAVEH at MACC.WISC.EDU
Tue Dec 28 18:20:38 EST 1993


> > Second does any have/use a buffer with T7 RNAP that gives higher yield
> > than mine ?
> 
> 
> Better tell us what your is, and what type of template you are using.
> 
> 
> Jim
> J. Graham

1) Standard BRL T7 RNAP and BRL buffer. Nothing special.

2) Picornaviral transcripts are being generated from a linearized template.
(vector clones are in is bluescribe)

Dave  



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