Increased Transcription Yields

[Dave Hall]

> > Second does any have/use a buffer with T7 RNAP that gives higher yield
> > than mine ?
> 
> 
> Better tell us what your is, and what type of template you are using.
> 
> 
> Jim
> J. Graham

1) Standard BRL T7 RNAP and BRL buffer. Nothing special.

2) Picornaviral transcripts are being generated from a linearized template.
(vector clones are in is bluescribe)

Dave