Preferential insert orientation

Jonathan Gary jgary at ewald.mbi.ucla.edu
Wed Dec 29 16:47:43 EST 1993


In article <CItDDr.1GH at ncifcrf.gov> Paul N Hengen,
pnh at fcs260c2.ncifcrf.gov writes:
>
>1. Secondary structure of the DNA fragments may prohibit the ligation in
one
>particular orientation, ie., one end of each molecule is much more
frequently
>ligated first.
>
>2. The transformants of both types of molecules are never seen because
the
>insert may be expressed from a vector promoter in only one orientation
and the
>product(s) are lethal to the cell.
>
>**********************************************************************
********
>* Paul N. Hengen, Ph.D.                          
/--------------------------/*
>* National Cancer Institute                       |Internet:
pnh at ncifcrf.gov |*
>* Laboratory of Mathematical Biology              |   Phone: (301)
846-5581  |*
>* Frederick Cancer Research and Development Center|     FAX: (301)
846-5598  |*
>* Frederick, Maryland 21702-1201 USA             
/--------------------------/*
>**********************************************************************
********


THERE you have it folks the President to be has spoken!!!!!!!!!!!!!!!   
:-)

(btw Paul -Dr. Hengen- nice article in TIBS)



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        Jonathan D. Gary                      Savoir Faire
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