Preferential insert orientation

Tim Buss tbuss at fred.fhcrc.org
Wed Dec 29 13:22:03 EST 1993


Re: Preferential insert orientation

I agree with Peter. This promoter is 'leaky' and there will be a low
level of expression even in the un-induced state. If your protein is at
all toxic to the cell you will tend to only get clones in the reverse
orientation.
I work with immunoglobulins and try to avoid using vectors of this type
for this very reason. 

Consider using a vector with a different promoter.



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