Elimination of the fixing step in sequencing

Harry Harry
Wed Dec 29 10:48:59 EST 1993


In article <2fn2qg$kg1 at news.mic.ucla.edu> Jonathan Gary <jgary at ewald.mbi.ucla.edu> writes:
>To: Hinayana Bawagan
>
>I have *never* fixed my sequencing gels.... We use Long Ranger Acryl. mix
>here and we go directly from pulling the plates apart right onto the gel
>drier!! :-)
>Fixing is really only nec. when you are running seq. gels with wedged
>spacers - the fixing gives some added rigidity to the thinner upper
>portion of the gel.
>As for stickyness, we just add a thin coat of J&J baby powder to the
>dried gel, this stops any left over tackyness after drying (and prevents
>ghost blobs on the film from static sparks as you peel off the film).
>Hope this helped..................................


I have heard before that not fixing the gel can lead to damage to the
gel drier pump from the urea.  Is there any truth to this?



More information about the Methods mailing list