Sequencing Gel Drying hardware - Experiences?

Leonard N. Bloksberg bloksber at pilot.msu.edu
Thu Dec 30 15:20:00 EST 1993


In Article <CIv6rx.JKE at knot.ccs.queensu.ca> "Peter T. Boag <BOAGP at QUCDN.QUEENSU.CA>" says:
> After a number of years of drying our sequencing gels directly onto
> glass plates and exposing them to film sandwiched onto the glass, we
> have become tired of periodic problems of gels not sticking to the
> "sticky" plate, or not releasing from the "nonsticky" plate. Most of
> the time it works ok, but it is frustrating when it doesn't.	We are
> contemplating getting a gel drier for at least back-up use. People
> we have spoken to use everything from a plastic, $1000 Bio-Rad drier
> hooked up to an aspirator or house vacuum with only an unchilled
> Erlenmyer trap,  to a $5000, stainless steel, all in one Savant
> drier with special vacuum pump and integral trap. ANy experience
> with this type of equipment, obvious do's or don'ts would be
> appreciated, so we can put together a reliable system. Thanks in
> advance.
> ******************************************************************
> ********************
> 
> Peter T. Boag			TEL: (613) 545-6160
> Department of Biology		FAX: (613) 545-6617
> Queen's University			EMAIL: BOAGP at QUCDN.QUEENSU.CA
> Kingston, Ontario
> Canada  K7L3N6
> 
> ******************************************************************
> ********************
> 
The simpler, the better.  Here is a place where IMHO the fancier models
are no better than the simplest, and may be more complicated to operate.
Do be sure to measure the drying platten, that it is an inch larger than
your gels on all sides.
			Leonard N. Bloksberg
.



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