Methylene Blue DNA staining protocol

Helge Weissig helgew at
Thu Dec 30 14:53:39 EST 1993

btw - MB works for RNA blots as well. I generally use it to stain the
marker lane after transfer onto nylon (Hybond-N) by soaking 0.04% MB, 0.5 M
NaOAc, pH 5.2 for 5 min. Brief rinsing w/ H2O usually reveils nice bands
with eg. 4 ul BRL 0.24-9.5 kb RNA ladder....

my 2 ul worth

PS. a little belated since the e-mail to news does not work :))


Helge Weissig      

La Jolla Cancer Research Foundation             (619) 455 6480 x253
10901 N. Torrey Pines Rd.                  FAX: (619) 455 0181
La Jolla, CA 92034                      e-mail: helgew at


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