Re-using Northern Blots?

Daniel Meier dmeier at post.its.mcw.edu
Mon Feb 1 17:30:24 EST 1993


k_garson at icrf.icnet.uk wrote:
: 	
: I would like to hear suggestions for mutiple probing of Northern
: blots. I have been using Hybond N+ and have had good results with
: the first blot, but poor results when I have attempted to reprobe. I
: have been stripping the probe by immersing the membrane in water
: at 90-95 degrees C. My problem is not stripping the probe, but
: getting the membrane to hybridize with new probe. Any suggestions?
: 
: Ken Garson
: ICRF
: London, UK



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