Re-using Northern Blots?

Daniel Meier dmeier at
Mon Feb 1 17:30:24 EST 1993

k_garson at wrote:
: I would like to hear suggestions for mutiple probing of Northern
: blots. I have been using Hybond N+ and have had good results with
: the first blot, but poor results when I have attempted to reprobe. I
: have been stripping the probe by immersing the membrane in water
: at 90-95 degrees C. My problem is not stripping the probe, but
: getting the membrane to hybridize with new probe. Any suggestions?
: Ken Garson
: London, UK

More information about the Methods mailing list