What form of Glycyl Betaine for protein expression

J Preiss--Seq Anal preissj at CLVAX1.CL.MSU.EDU
Fri Feb 5 16:09:00 EST 1993


Hi Netters

	A short while ago there was a discussion about expressing fussion
proteins in E. coli in which several people recomended using 25 dC growth 
temp and Sorbitol and Glycyl Betaine in the medium as described in the 
Blackwell and Horgan paper (FEBS, 1991, 245(1,2,3)10-12).  I want to try 
it, but there are several forms of Glycyl Betaine listed in Sigma (the 
source cited in the paper).  In the Sigma catalog, page 159, should I use
#B-2629 (free base, anhydrous), #B-2754 (free base, monohydrate), or #B-3501
(hydrochloride)? 

	Thanks

	Lenny Bloksberg
	PreissJ at clvax1.cl.msu.edu
	Dept. of Biochemistry
	Michigan State University

ps  I know I could just call them, but the net is just so much fun.  :-)




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