Pulsed Field Electrophoresis

Shaun D. Black SHAUN%JASON.DECNET at relay.the.net
Fri Feb 5 12:28:29 EST 1993


In answer to Sonya Clark's question on pulsed-field electrophoresis: 


*Pertinent Terminology and Abbreviations:

     AFIGE     Asymmetric Field Inversion Gel Electrophoresis
     CFE       Crossed Field Electrophoresis
     CHEF      Contour Clamped Hopmogeneous Electric Field
     FIGE      Field Inversion Gel Electrophoresis
     OFAGE     Orthogonal Field Alternation Gel Electrophoresis
     PACE      Programmable Autonomously Controlled Electrode Gel Electroph   
     PFGE      Pulsed-Field Gradient Electrophoresis or P- F- Gel E-)
     PHOGE     Pulsed Homogeneous Orthogonal Field Gel Electrophoresis
     RGE       Rotating Gel Electrophoresis
     TAFE      Transverse Alternating Field Electrophoresis 


*References: (Not exhaustive, but ones I have found useful when learning 
about PFGE)

     "Megabase Scale Mapping of the HLA Gene Complex by Pulsed Field Gel 
Electrophoresis" Laweance, S.K., Smith, C.L., Srivastava, R., Cantor, C.R., 
and Weissman, S.M. (1987) Science 235: 1387-1390.

     "A Novel Instrument for Separating Large DNA Molecules with Pulsed 
Homogenouse Electric Fields" Clark, S.M., Lau, E., Birren, B.W, and Hood,
L. (1988) Science 241: 1203-121205.

     "Transverse Alternating Field Electrophoresis (TAFE)" Stewart, G., 
Furst, A., and Avdalovic, N. (1988) BioTechniques 6: 68-74.

     "Pulsed-Field Gel Electrophoresis electrifies Genetic Research" Roberts,
S.S. (1989) J. NIH Res. 1: 113-114.

     "A Megabase DNA ELectrophoresis System" Ferris, S., Freeby, S., Zoller,
P., Ragsdale C., and Stevens, A. (1989) Am. Biotech. Lab. January 36-41.

     "Pulsed Field Gel Electrophoresis" Lai, E., Birren, B.W., Clark, S.M., 
Simon, M.I., and Hood, L. 1989) BioTechniques 7: 34-42. (Primary references 
cited)

     "Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis.
A Review of the Basic Phenomenology" Olson, M.V. (1989) J. Chromatogr. 
470: 377-383.


*Commercial Suppliers of Apparatuses: (Not necessarily exhaustive)

     Baekon, Inc. (Saratoga, CA)
     Beckman Instruments (Fullerton, CA)
     BioRad Laboratories (Richmond, CA)
     CBS Scientific Company (Del Mar, CA)
     Dan-Kar Corp. (Reading, MA)
     EC Apparatus Corp (St. Petersburg, FL)
     Hoefer Scienfific Instruments (San Francisco, CA)
     Life Technologies, Inc. (Grand Island, NY)
     Owl Scientific Plastics (Cambridge, MA)
     P&S Biochemicals, Inc. (Gaithersburg, MD)
     Pharmacia LKB Biotechnology, Inc. (Piscataway, NJ)
     West Coast Scientific, Inc. (Hayward, CA)

I hope this helps.  Cheers!  :-)  -Shaun

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  = Shaun D. Black, PhD     | Internet:  shaun%jason.decnet at relay.the.net = 
  = Dept. of Biochemistry   | Bitnet:    shaun%jason.decnet at thenic.bitnet = 
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