vmiao at oregon.uoregon.edu
Fri Feb 5 03:09:55 EST 1993
In article <C1wr6A.J05 at bunyip.cc.uq.oz.au>, galileo
<galileo at biosci.uq.edu.au> wrote:
> In article <1993Feb1.200728.17450 at cc.umontreal.ca> Coady Michael,
> coady at ERE.UMontreal.CA writes:
> > I know practically zero about pulsed field gel electrophoresis,
> >but I noticed that Hoefer is selling one apparatus that they say is
> useful for relatively small DNAs.
> G'day mate,
> Welcome to the world of PFGE. I have done a fair amount and I still feel
> that I know nothing. However, I would not use PFGE to separate DNA
> fragments under 20 kb but a standard agarose gel. Good luck with your
> C. Dundee.
> galileo at florey.biosci.uq.oz.au
I agree - PFGE would certainly work, but standard elelctrophoresis
should be fine for fragments under 20 kb. I routinely use 0.6%
agarose gels, and once in a while even run a 0.4% agarose gel (pour
a thin 1% agarose layer for support if you need to handle the gel
eventually, this layer shouldn't touch the bottom of the comb -then
pour the 0.4% gel while it is hot, so that it forms a good seal with
the bottom layer).
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