A question about subcloning

Anthony Davies adavies at molbiol.ox.ac.uk
Sat Feb 6 10:29:58 EST 1993


In article <930202121943.b43d at VTHVAX.TAMU.EDU>, MCAO at VTHVAX.TAMU.EDU writes:
> Hi, I plan to subclone several of my cDNA into expression vector in baculovirus
> system and express them. All of my clone have a 3' non-coding region and poly-A
> tail. Is it better to include this sequence or leave it out? What other traps
> I should pay attention to during this process? Thanks in advance.
> 					Ming Cao

Almost all baculovirus expression vectors use the polyhedrin gene terminator 
and polyA site, which itself appears to contribute significantly to the high 
level of expression you will see - so stop your insert at the stop codon.

Anthony Davies
Institute of Virology
Oxford UK



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