pSV2pac plasmid wanted
francis at monod.Biol.McGill.CA
Sun Feb 7 18:38:56 EST 1993
pnj at stein.u.washington.edu (JnP) writes:
> Can anybody tell me where to obtain a pSV2pac plasmid or
>just a plasmid that contains the 'puromycin N-acetyl transferase'
>gene that encodes puromycin resistance, in the US? The gene was
>originally cloned by Dr. Antonio Jimenez's group in Madrid, Spain.
>But I cannot find their telephone or Fax #. If anyone knows this
>info, could you please share with me? I would appreciate every
>help I could get. Thank!
using gopher (discussed _many many_ times in this and other bionet/BIOSCI
groups) I found the gene 'puromycin N-acetyl transferase', but not the
pSV2pac you mention. If you want to know more about Gopher check the
bionet/BIOSCI FAQ (frequently asked question) info sheet ... if you still
need help, give me an Email ...
LOCUS S73283 1332 bp DNA BCT 24-APR-1992
DEFINITION pac=puromycin N-acetyltransferase, dmpM=O-demethyl puromycin
O-methyltransferase [Streptomyces alboniger, Genomic, 1332 nt]
(I sent the whole file to Fred under seperate cover)
| B.F. Francis Ouellette
| manager, yeast chromosome I & XVI sequencing project
| dept of biology, McGill university, Montreal, Qc, Canada
| francis at monod.biol.mcgill.ca
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