Releasing adherent cells w/o trypsin

[bma14432 at vax1.utulsa.edu]

Dear Netter,

I vaguely recall a conversation a couple years ago where someone
mentioned a protocol for releasing adherent cells from tissue culture
flasks/dishes without trypsinizing them. I believe it involved EDTA but
I'm not sure. If anyone has a reference or protocol for this I would
be very appreciative. My main concern is to release the cells with
minimal disturbance to the membrane. If you have other ideas please feel
free to suggest them. Thanks in advance.
 


Mark A. Brudnak
University of Tulsa
BMA14432 at VAX1.UTULSA.EDU
All opinions expressed are MINE