Biotinylated probes

Geoff Vince vince at
Mon Feb 8 16:22:26 EST 1993

Can anyone provided a tried and tested protocol for biotinylating cDNA 
oligonucleotide probes.  I have inherited a rough protocol which uses 
terminal transferase, Bio-dUTP, mercaptoethanol, dCTP, and Cacodylate 
buffer, although the concentrations of the solutions appear to be dubious. 



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