mRNA isolation fron bacteria

Cornelius Krasel zxmkr08 at studserv.zdv.uni-tuebingen.de
Tue Feb 9 10:07:28 EST 1993


In <CMM.0.88.728441416.malam at uhunix.uhcc.Hawaii.Edu> malam at UHUNIX.UHCC.HAWAII.EDU (Maqsudul Alam) writes:

>Can anybody help us to isolate mRNA from bacterial cells? What is the best
>method or kit available in the market?

I work with the "hot phenol" method which has been described in:
D. Georgellis, S. Arvidson, A. v. Gabain, J. Bacteriol. 174(1992),5382-5390.
This method works fine for me. Basically, bacteria are suspended in 2% SDS
and extracted with hot phenol (65 C).
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/* Cornelius Krasel, Department of Physiological Chemistry, U Tuebingen    */ 
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